What is the clinical evidence from this study?

Study design: Other. Intervention: Shear stress. Primary outcome: ACE activity and mRNA expression (p=< 0.0001).

What question did this study set out to answer?

To investigate the effects of hemodynamic shear stress on angiotensin converting enzyme (ACE) expression in vascular cells, focusing on regulatory pathways in smooth muscle cells.

January 14, 2000Cardiovascular ResearchOpen Access

Shear stress induces angiotensin converting enzyme expression in cultured smooth muscle cells: possible involvement of bFGF

Q: What are the key findings of this study?

A 10 dynes/cm2 shear stress for 24 hours significantly increased ACE activity and mRNA expression in cultured rat vascular cells, potentially mediated by bFGF (P<0.0001 for shear intensity dependence).

Q: What question did this study set out to answer?

To investigate the effects of hemodynamic shear stress on angiotensin converting enzyme (ACE) expression in vascular cells, focusing on regulatory pathways in smooth muscle cells.

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Key result

A 10 dynes/cm2 shear stress for 24 hours significantly increased ACE activity and mRNA expression in cultured rat vascular cells, potentially mediated by bFGF (P<0.0001 for shear intensity dependence).

Population

Cultured rat aortic endothelial cells, smooth muscle cells, and fibroblasts isolated from Wistar rats

Comparison

Shear stress using a laminar shear flow parallel… vs Unstressed cells

Design

Preclinical

Follow-up

24 hours

Authors

WGWilly GosgnachUniversity of Catania

Discussion

Member takes

Implication

Hypothesis-generating for shear-mediated vascular ACE regulation; leaves open human relevance and therapeutic implications.

Key Points

To investigate the effects of hemodynamic shear stress on angiotensin converting enzyme (ACE) expression in vascular cells, focusing on regulatory pathways in smooth muscle cells.
Cultured rat aortic endothelial cells, smooth muscle cells, and fibroblasts isolated from Wistar rats were exposed to laminar shear stress using a parallel flow chamber.
Measured ACE enzymatic activity, ACE mRNA levels, and basic fibroblast growth factor (bFGF) mRNA under varying shear intensities and durations, with and without cycloheximide (10^-4 M) translation inhibition.
A 24-hour shear rate of 10 dynes/cm2 increased ACE activity across all cell types (2.14-fold in endothelial cells, 2.9-fold in smooth muscle cells, and 3.33-fold in fibroblasts), which was completely blocked by cycloheximide pre-incubation.
In smooth muscle cells, ACE mRNA elevation was dependent on shear intensity (P < 0.0001); 6 hours of 15 dynes/cm2 shear did not alter ACE expression but significantly increased bFGF mRNA by 3.7-fold.
Direct treatment with bFGF dose-dependently stimulated ACE mRNA expression and enzymatic activity in smooth muscle cells.

Structured PICO

Population

In vitro study using cultured rat aortic endothelial cells, smooth muscle cells, and fibroblasts isolated from Wistar rats.

Exposure

Shear stress (10 dynes/cm2 for 24 h, or 15 dynes/cm2 for 6 h) using a laminar shear flow parallel chamber

Comparator

Unstressed cells

Outcome

ACE activity and mRNA expressionsurrogate

Main Result

p-value: p=< 0.0001

Mechanical shear stress increases ACE expression in vascular cells, potentially mediated by bFGF, suggesting a mechanism for vascular wall remodeling.

Cite This Study

Willy Gosgnach (2000) studied this question. Shear stress was evaluated on ACE activity and mRNA expression (p=< 0.0001). A 10 dynes/cm2 shear stress for 24 hours significantly increased ACE activity and mRNA expression in cultured rat vascular cells, potentially mediated by bFGF (P<0.0001 for shear intensity dependence).

synapsesocial.com/papers/6a96af792af794539aa6c119 https://doi.org/10.1016/s0008-6363(99)00269-2

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Shear stress induces angiotensin converting enzyme expression in cultured smooth muscle cells: possible involvement of bFGF

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Shear stress induces angiotensin converting enzyme expression in cultured smooth muscle cells: possible involvement of bFGF

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Cite This Study

Also Consider

Also Consider