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December 1, 1991Journal of Cardiac SurgeryOpen Access

Immunogenicity, Antigenicity, and Endothelial Viability of Aortic Valves Preserved at 4°C in a Nutrient Medium

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Why the study?

Does prolonged storage at 4°C in a nutrient medium alter the antigenicity, immunogenicity, and endothelial viability of rat aortic valve allografts?

Population

Rat aortic valves (from Brown Norway rats, implanted heterotopically into the abdominal aorta of Lewis rats)

Comparison

Storage at 4°C in RPMI containing 10% fetal calf… vs Fresh valves and valves stored for shorter…

Design

Preclinical

Follow-up

up to 21 days (storage duration)

Authors

FLFlavian M. LupinettiUniversity of IowaJCJeffrey P. ChristyDKDavid M. KingMedical College of Wisconsin

Discussion

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Overview

Prolonged storage should not yet change clinical allograft practices; hypothesis-generating for optimized preservation to minimize residual immunogenicity.

Structured PICO

Does prolonged storage at 4°C in a nutrient medium alter the antigenicity, immunogenicity, and endothelial viability of rat aortic valve allografts?

P
Population
Rat aortic valves (from Brown Norway rats, implanted heterotopically into the abdominal aorta of Lewis rats)
I
Intervention
Storage at 4°C in RPMI containing 10% fetal calf serum for up to 3 weeks following antibiotic sterilization
C
Comparator
Fresh valves and valves stored for shorter durations (e.g., 1 day)
O
Outcome
Antigenicity (Class I and II antigens), immunogenicity (duration of second-set skin graft survival), and endothelial cell viabilitysurrogate

Prolonged storage of aortic valve allografts at 4°C reduces endothelial viability and attenuates, but does not eliminate, immunogenicity and antigenicity.

Cite This Study

Lupinetti et al. (1991) studied this question.

synapsesocial.com/papers/6a96cfbd8a4936f1ceb30241https://doi.org/10.1111/j.1540-8191.1991.tb00345.x
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Also Consider

Synapse has enriched one closely related paper. Consider it for comparative context:

  1. 1Long-term follow-up of patients with the antibiotic-sterilized aortic homograft valve inserted freehand in the aortic position.1987 · 202 citations