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October 25, 2016Journal of Translational MedicineOpen Access

Recombinant Ang-2 treatment significantly impaired blood flow recovery and reduced collateral arteriole diameter in a mouse model of hindlimb ischaemia.

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Why the study?

Does recombinant Angiopoietin-2 impair collateral artery growth and blood flow recovery in a mouse model of hindlimb ischaemia?

Population

Male C57BL/6 mice subjected to unilateral hindlimb ischaemia by femoral artery ligation

Comparison

Recombinant human Ang-2 injected… vs Vehicle injected intraperitoneally every other day

Design

Preclinical

Follow-up

7 days

Key result

Recombinant Ang-2 treatment significantly impaired blood flow recovery and reduced collateral arteriole diameter in a mouse model of hindlimb ischaemia.

Authors

XTXiaoyong TanKYKai YanMRMeiping Ren

Discussion

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Member takes

Overview

Should not change clinical practice; hypothesis-generating for Ang-2 targeting to promote arteriogenesis in ischaemia.

Structured PICO

Does recombinant Angiopoietin-2 impair collateral artery growth and blood flow recovery in a mouse model of hindlimb ischaemia?

P
Population
Male C57BL/6 mice subjected to unilateral hindlimb ischaemia to evaluate the effects of recombinant Ang-2 on collateral artery growth over 7 days.
I
Intervention
Recombinant human Ang-2 (50 µg/mL) injected intraperitoneally every other day beginning 1 day before surgery
C
Comparator
Vehicle (PBS) injected intraperitoneally every other day
O
Outcome
Blood flow perfusion (ratio of ischaemic to normoxic limb) measured using a laser Doppler perfusion imagersurrogate

Main Result

p-value: p=<0.05

In a mouse model of hindlimb ischaemia, Angiopoietin-2 impairs collateral artery growth and blood flow recovery by suppressing macrophage infiltration and downregulating angiogenic factors.

Limitations

  • Cannot definitively determine if the inhibitory effect of Ang-2 on arteriogenic activity depends on macrophages compared with pericytes.
  • Only detected the effect of Ang-2 at an early time point (7 days).
  • Late adaptive responses to hind limb ischaemia were not assessed.
  • Cannot definitively determine if the inhibitory effect of Ang-2 on arteriogenic activity depends on macrophages compared with pericytes
  • Only detected the effect of Ang-2 at an early time point (7 days)

Cite This Study

Tan et al. (2016) studied Hindlimb ischaemia. Recombinant human Ang-2 vs. Vehicle (PBS) was evaluated on Blood flow recovery (ratio of ischaemic to non-ischaemic hindlimb blood flow) (p=<0.05). Recombinant Ang-2 treatment significantly impaired blood flow recovery and reduced collateral arteriole diameter in a mouse model of hindlimb ischaemia.

synapsesocial.com/papers/6a973005691dfef81e3db35ahttps://doi.org/10.1186/s12967-016-1055-x
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Angiopoietin-2 Impairs Revascularization After Limb Ischemia2007 · 112 citations
  2. 2Angiopoietin-2 Stimulates Blood Flow Recovery After Femoral Artery Occlusion by Inducing Inflammation and Arteriogenesis2008 · 62 citations
  3. 3Angiopoietin-2 Is Critical for Cytokine-Induced Vascular Leakage2013 · 179 citations
  4. 4Early Vessel Destabilization Mediated by Angiopoietin-2 and Subsequent Vessel Maturation via Angiopoietin-1 Induce Functional Neovasculature after Ischemia2013 · 45 citations
  5. 5Reciprocal regulation of angiopoietin-1 and angiopoietin-2 following myocardial infarction in the rat*12004 · 60 citations