What question did this study set out to answer?

To determine the relationship between cell volume, intracellular pH, basolateral membrane potential, and basolateral ion conductances during hypotonic shock in proximal convoluted tubules.

February 1, 1993AJP Renal Physiology

Basolateral K+, Cl-, and HCO3- conductances and cell volume regulation in rabbit PCT

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Authors

PMPaolo MacrìUniversity of Catania SBSylvie BretonHôtel-Dieu de Québec JBJ. S. BeckUnilever (United Kingdom)

Discussion

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Implication

Laboratory study reveals basolateral chloride and potassium conductances drive cell volume regulation in rabbit proximal tubules, indicating chloride conductance is the rate-limiting step.

Key Points

To determine the relationship between cell volume, intracellular pH, basolateral membrane potential, and basolateral ion conductances during hypotonic shock in proximal convoluted tubules.
Subjected collapsed rabbit proximal convoluted tubules to a 125-mosmol/kg hypotonic shock to stimulate rapid swelling and subsequent regulatory volume decrease.
Measured changes in basolateral membrane potential (VBL), intracellular pH (pHi), and partial and absolute membrane conductances for potassium, chloride, and the sodium-bicarbonate cotransporter.
Hypotonic shock provoked an immediate hyperpolarization of VBL by 10.0 ± 1.5 mV followed by a secondary hyperpolarization of 5.0 ± 0.7 mV from a baseline of -44.0 ± 4.6 mV, with pHi shifting transiently from 7.09 ± 0.03 to 7.24 ± 0.05.
Absolute membrane conductance for potassium increased at least 8-fold and chloride conductance increased at least 17-fold, while sodium-bicarbonate cotransporter conductance increased only slightly.
The rapid rise and subsequent decline in chloride conductance closely mirrored the time course of cellular water efflux, identifying chloride transport as the rate-limiting step for volume recovery.

Cite This Study

Macrì et al. (1993) studied this question.

synapsesocial.com/papers/6a97432ec3caee290889aaac https://doi.org/10.1152/ajprenal.1993.264.2.f365

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Implication

Laboratory study reveals basolateral chloride and potassium conductances drive cell volume regulation in rabbit proximal tubules, indicating chloride conductance is the rate-limiting step.

Basolateral K+, Cl-, and HCO3- conductances and cell volume regulation in rabbit PCT

Discussion

Member takes

Implication

Key Points

Cite This Study

Basolateral K+, Cl-, and HCO3- conductances and cell volume regulation in rabbit PCT

Discussion

Member takes

Implication

Key Points

Cite This Study

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