September 1, 1972The Journal of Immunology
Radioimmunoassay of Mammalian Type C Viral Proteins
Key result
A double antibody VP3(gs) antigen radioimmune precipitation assay was at least 500 times more sensitive than complement-fixation or immunodiffusion for quantitating mammalian type C viral proteins.
Population
Murine and feline type C viruses (VP3(gs) protein)
Comparison
Radioimmune precipitation assay using… vs Complement-fixation or immunodiffusion
Design
Preclinical
Authors
Discussion
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Implication
Advances sensitive type C viral protein detection in animal models; leaves open translation to human diagnostics.
Structured PICO
P
Population
Murine and feline type C viruses (VP3(gs) protein)
E
Exposure
Radioimmune precipitation assay (RIPA) using 125I-labeled VP3(gs)
C
Comparator
Complement-fixation or immunodiffusion
O
Outcome
Sensitivity and specificity of the assay for quantitating unlabeled murine or feline VP3(gs) antigensurrogate
Main Result
Effect estimate: at least 500 times more sensitive
The developed radioimmune precipitation assay provides a highly sensitive and specific method for detecting and quantitating mammalian type C viral proteins.
Cite This Study
Scolnick et al. (1972) studied Mammalian type C viruses. Radioimmune precipitation assay (RIPA) vs. Complement-fixation or immunodiffusion was evaluated on Assay sensitivity and specificity (at least 500 times more sensitive). A double antibody VP3(gs) antigen radioimmune precipitation assay was at least 500 times more sensitive than complement-fixation or immunodiffusion for quantitating mammalian type C viral proteins.