Key result
The DNA sequence corresponding to the hepatitis C virus 5' UTR exhibits strong promoter activity, initiating mRNA at nucleotide 67, which was not seen with HCV 3' UTR or poliovirus 5' UTR.
Population
Stably transfected cells and DNA constructs
Comparison
DNA sequence corresponding to the hepatitis C… vs HCV 3' UTR or poliovirus 5' UTR sequences
Design
Preclinical
Authors
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Bicistronic HCV reporter data require transcriptional controls; leaves open in vivo relevance for viral expression studies.
The DNA sequence corresponding to the HCV 5' UTR exhibits strong promoter activity, suggesting that data from bicistronic DNA constructs carrying this sequence should be analyzed at both translational and transcriptional levels.
Estelle Dumas-Mallet (2003) studied Hepatitis C virus (HCV). DNA sequence corresponding to the HCV 5' UTR vs. HCV 3' UTR or poliovirus 5' UTR sequences was evaluated on Promoter activity and mRNA initiation. The DNA sequence corresponding to the hepatitis C virus 5' UTR exhibits strong promoter activity, initiating mRNA at nucleotide 67, which was not seen with HCV 3' UTR or poliovirus 5' UTR.
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