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October 1, 1998Journal of General Virology

Translation efficiencies of the 5' untranslated region from representatives of the six major genotypes of hepatitis C virus using a novel bicistronic reporter assay system.

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Population

Four cell lines: BHK-21, HeLa-T4+, HuH7 and HepG2 transfected with bicistronic, dual luciferase reporter…

Comparison

5' untranslated region from different HCV… vs Comparison between different HCV genotypes

Design

Preclinical

Authors

ACAmy CollierEdinburgh Royal InfirmarySTShixing TangSouthern Medical UniversityRERebecca ElliottUniversity of Manchester

Discussion

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Implication

Does not support changes in HCV care; leaves open the role of 5'UTR variation in disease progression.

Structured PICO

P
Population
Four cell lines: BHK-21, HeLa-T4+, HuH7 and HepG2 transfected with bicistronic, dual luciferase reporter constructs containing 5' regions of HCV isolates representing the six major genotypes.
I
Intervention
5' untranslated region (5'UTR) from different HCV genotypes
C
Comparator
Comparison between different HCV genotypes (e.g., genotype 2b vs 6a)
O
Outcome
Relative expression of two luciferases (HCV IRES-directed vs cap-dependent ribosome scanning)surrogate

HCV isolates from different genotypes do not translate their open reading frames with equal efficiency, with genotype 2b showing the highest efficiency in vitro.

Cite This Study

Collier et al. (1998) studied this question.

synapsesocial.com/papers/6a82136971d4e96905f1d08dhttps://doi.org/10.1099/0022-1317-79-10-2359
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Translation of human hepatitis C virus RNA in cultured cells is mediated by an internal ribosome-binding mechanism1993 · 444 citations
  2. 2Stability of a stem-loop involving the initiator AUG controls the efficiency of internal initiation of translation on hepatitis C virus RNA.2020 · 252 citations
  3. 3Picornavirus internal ribosome entry segments: comparison of translation efficiency and the requirements for optimal internal initiation of translation<i>in vitro</i>1995 · 151 citations
  4. 4Complete nucleotide sequence of a type 4 hepatitis C virus variant, the predominant genotype in the Middle East.1997 · 204 citations
  5. 5Variation of the hepatitis C virus 5' non-coding region: implications for secondary structure, virus detection and typing1995 · 229 citations