August 1, 2000Journal of VirologyOpen Access

Cell Type-Specific Enhancement of Hepatitis C Virus Internal Ribosome Entry Site-Directed Translation due to 5′ Nontranslated Region Substitutions Selected during Passage of Virus in Lymphoblastoid Cells

View Full Paper

Ask AI

Bookmark

Population

Human lymphoblastoid cell lines, monocyte/granulocyte cell lines, hepatocyte cell lines, and rabbit…

Comparison

Dicistronic RNAs containing upstream and… vs IRES present in the dominant H77 quasispecies

Design

Preclinical

Authors

HLHervé LeratInserm YSYoko ShimizuKyoto University SLStanley M. LemonUniversity of North Carolina at Chapel Hill

Discussion

Member takes

Implication

Should not change clinical practice; leaves open whether these IRES variants drive HCV compartmentalization in vivo.

Structured PICO

Population

Human lymphoblastoid cell lines (Raji, Bjab, Molt4, Jurkat, HPBMa10-2), monocyte/granulocyte cell lines (HL-60, KG-1, THP-1), hepatocyte cell lines (Huh-7), and rabbit reticulocyte lysates

Intervention

Dicistronic RNAs containing upstream and downstream reporter sequences separated by HCV IRES sequences with three specific nucleotide substitutions (G(107)-->A, C(204)-->A, and G(243)-->A)

Comparator

IRES present in the dominant H77 quasispecies (wild-type)

Outcome

Translational activity (relative firefly and Renilla luciferase activities)surrogate

Specific nucleotide substitutions in the HCV 5'NTR enhance IRES-directed translation specifically in certain lymphoblastoid cells, potentially explaining viral quasispecies differences between liver and peripheral blood mononuclear cells.

Cite This Study

Lerat et al. (2000) studied this question.

synapsesocial.com/papers/6a8be19eeffeb02058b1ed68 https://doi.org/10.1128/jvi.74.15.7024-7031.2000

View Full Paper

Ask AI

Bookmark

Cell Type-Specific Enhancement of Hepatitis C Virus Internal Ribosome Entry Site-Directed Translation due to 5′ Nontranslated Region Substitutions Selected during Passage of Virus in Lymphoblastoid Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Cell Type-Specific Enhancement of Hepatitis C Virus Internal Ribosome Entry Site-Directed Translation due to 5′ Nontranslated Region Substitutions Selected during Passage of Virus in Lymphoblastoid Cells

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider