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September 2, 2026Combinatorial Chemistry & High Throughput Screening

Therapeutic Mechanism of Huayu Zhitong Plaster in Acute Soft Tissue Injury Rats via the PI3K/Akt/NF-κB Signaling Pathway

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Authors

XLXiaohui LiuYFYajin FengLPLixin Peng

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Overview

Preclinical study shows that topical Huayu Zhitong Plaster relieves inflammation and tissue damage in rats, highlighting therapeutic repair via PI3K/Akt/NF-κB pathway inhibition.

Key Points

  • Investigate the therapeutic efficacy and underlying molecular mechanism of Huayu Zhitong Plaster in a rat model of acute soft tissue injury.
  • Profiled the chemical composition of Huayu Zhitong Plaster using UPLC-Q-TOF-MS/MS and predicted therapeutic targets via network pharmacology.
  • Established an acute soft tissue injury model in rats through blunt trauma to the gastrocnemius muscle.
  • Assessed tissue histology via H&E staining, inflammatory and oxidative markers via ELISA, and signaling protein expression via immunofluorescence and Western blotting.
  • Identified 86 active constituents, with network pharmacology predicting target convergence on the PI3K-Akt and NF-κB pathways.
  • Huayu Zhitong Plaster significantly suppressed phosphorylated PI3K, Akt, and NF-κB expression in injured muscle tissue.
  • Treatment reduced inflammatory markers IL-6 and PGE2, lowered MDA and MPO, elevated antioxidant SOD activity, and markedly decreased edema and inflammatory cell infiltration.

Cite This Study

Liu et al. (2026) studied this question.

synapsesocial.com/papers/6a97e305c562ede874ec7a00https://doi.org/10.2174/0113862073450412260807073516
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