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January 1, 1979Journal of VirologyOpen Access

Inhibition of coronavirus 229E replication by actinomycin D

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Why the study?

Does actinomycin D inhibit the replication of human coronavirus 229E in L132 cells?

Population

L132 cells infected with human coronavirus 229E

Comparison

Actinomycin D vs Untreated control cells (no drug)

Design

Preclinical

Follow-up

up to 48 hours post-infection

Authors

DKDouglas A. KennedyNational Heart Lung and Blood InstituteCJC. Margaret Johnson-LussenburgUniversity of Ottawa

Discussion

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Overview

Actinomycin D may inhibit early HCoV-229E replication in vitro; leaves open host-factor requirements for coronaviruses in vivo.

Structured PICO

Does actinomycin D inhibit the replication of human coronavirus 229E in L132 cells?

P
Population
L132 cells infected with human coronavirus 229E
I
Intervention
Actinomycin D (various concentrations, 50% inhibitory dose 0.1 µg/ml)
C
Comparator
Untreated control cells (no drug)
O
Outcome
Virus yield (replication of human coronavirus 229E)surrogate

Actinomycin D inhibits the early stages of human coronavirus 229E replication in L132 cells, suggesting a requirement for a limiting unstable host product.

Cite This Study

Kennedy et al. (1979) studied this question.

synapsesocial.com/papers/6a984eb6883af1d6c8a6a06bhttps://doi.org/10.1128/jvi.29.1.401-404.1979
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