July 1, 1991Water Science & Technology
The Application of a Poliovirus cDNA Probe for the Detection of Enteroviruses in Water
Key result
A dot blot assay using poliovirus cDNA probes was as sensitive as cell culture for the detection of enteroviruses in sewage-contaminated groundwater within 48 hours.
Why the study?
Does a poliovirus cDNA probe in a dot blot assay improve the detection of enteroviruses in water compared to cell culture?
Population
Water samples (sewage contaminated groundwater) and cell harvests
Comparison
Poliovirus cDNA probes labeled with [32P] dCTP… vs Cell culture
Design
Preclinical
Authors
Discussion
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Member takes
Implication
May enable faster enterovirus monitoring in groundwater; leaves open routine adoption pending further validation.
Structured PICO
Does a poliovirus cDNA probe in a dot blot assay improve the detection of enteroviruses in water compared to cell culture?
P
Population
Water samples (sewage contaminated groundwater) and cell harvests
I
Intervention
Poliovirus cDNA probes labeled with [32P] dCTP and [32P] dATP in a dot blot assay
C
Comparator
Cell culture
O
Outcome
Detection of enteroviruses/poliovirussurrogate
A dot blot assay using poliovirus cDNA probes provides a sensitive and rapid method for detecting enteroviruses in environmental water samples.
Cite This Study
Margolin et al. (1991) studied Enterovirus contamination in water. Poliovirus cDNA probe in a dot blot assay vs. Cell culture was evaluated on Detection of virus in sewage contaminated groundwater. A dot blot assay using poliovirus cDNA probes was as sensitive as cell culture for the detection of enteroviruses in sewage-contaminated groundwater within 48 hours.