What question did this study set out to answer?

To investigate whether sevoflurane preconditioning protects against cytosolic calcium loading, functional impairment, and infarction following prolonged hypothermic ischemia, and to evaluate the role of mitochondrial KATP channels in this cardioprotection.

October 1, 2002Anesthesiology

Sevoflurane Preconditioning before Moderate Hypothermic Ischemia Protects against Cytosolic Ca2+ Loading and Myocardial Damage in Part via Mitochondrial KATPChannels

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Why the study?

Does sevoflurane preconditioning improve cardiac function and reduce infarct size in guinea pig isolated hearts subjected to hypothermic ischemia?

Population

Guinea pig isolated hearts

Comparison

Sevoflurane preconditioning before 4-h global… vs Nontreated hypothermic ischemia

Design

Preclinical, randomly assigned

Authors

QCQun ChenKunming University of Science and Technology ACAmadou K.S. CamaraMedical College of Wisconsin JAJianzhong AnSuzhou University of Science and Technology

Discussion

Member takes

Implication

Sevoflurane preconditioning should not yet alter clinical practice; leaves open mitochondrial KATP-mediated cardioprotection in human hypothermic ischemia.

Key Points

To investigate whether sevoflurane preconditioning protects against cytosolic calcium loading, functional impairment, and infarction following prolonged hypothermic ischemia, and to evaluate the role of mitochondrial KATP channels in this cardioprotection.
Isolated guinea pig hearts were randomly assigned to three groups: untreated hypothermic ischemia control (CON), sevoflurane preconditioning with 3.5 vol% for 15 min and 15-min washout (SPC), or SPC bracketed with 200 µM of the mitochondrial KATP channel blocker 5-hydroxydecanoate (SPC + 5-HD).
All hearts were subjected to 4 h of global ischemia at 17°C followed by reperfusion.
Left ventricular cytosolic [Ca2+] was quantified using the fluorescent indicator indo-1 alongside measurements of cardiac mechanics, oxygen consumption, and infarct size.
Untreated control hearts exhibited marked increases in systolic and diastolic [Ca2+] upon reperfusion, coupled with depressed contractility, relaxation, oxygen consumption, and cardiac efficiency.
SPC hearts demonstrated reduced cytosolic [Ca2+] overloading (predominantly diastolic [Ca2+]), enhanced calcium influx/efflux kinetics, improved functional recovery and efficiency, and significantly smaller infarct size.
Coadministration of 5-HD directly antagonized the infarct-sparing protection conferred by SPC, while post-ischemic functional recovery was less markedly impaired.

Structured PICO

Does sevoflurane preconditioning improve cardiac function and reduce infarct size in guinea pig isolated hearts subjected to hypothermic ischemia?

Population

Guinea pig isolated hearts

Intervention

Sevoflurane preconditioning (3.5 vol% sevoflurane for 15 min with a 15-min washout) before 4-h global ischemia at 17 degrees C, with or without 200 microm 5-hydroxydecanoate (5-HD)

Comparator

Nontreated hypothermic ischemia (4-h global ischemia at 17 degrees C)

Outcome

Cardiac function, cytosolic [Ca2+] loading, and infarct size on reperfusionsurrogate

Sevoflurane preconditioning protects against myocardial damage during hypothermic ischemia in part via mitochondrial KATP channel opening.

Cite This Study

Chen et al. (2002) studied this question.

synapsesocial.com/papers/6a99b11775d50b594933a334 https://doi.org/10.1097/00000542-200210000-00025

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Implication

Sevoflurane preconditioning should not yet alter clinical practice; leaves open mitochondrial KATP-mediated cardioprotection in human hypothermic ischemia.

Sevoflurane Preconditioning before Moderate Hypothermic Ischemia Protects against Cytosolic Ca2+ Loading and Myocardial Damage in Part via Mitochondrial KATPChannels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Sevoflurane Preconditioning before Moderate Hypothermic Ischemia Protects against Cytosolic Ca2+ Loading and Myocardial Damage in Part via Mitochondrial KATPChannels

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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