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January 1, 2004Journal of Smooth Muscle ResearchOpen Access

Flow-induced endothelium-dependent vasoreactivity in rat mesenteric arterial bed

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Key result

High perfusion flow in rat mesenteric arterial beds increased perfusion pressure and stimulated the release of NO and ET-1, demonstrating that vascular tone is regulated by interacting endothelium-derived factors.

Population

Male Wistar rats aged 18 weeks (isolated mesenteric arterial bed preparation)

Comparison

Gradually increasing perfusion flow rates to… vs Low flow rate (5 ml/min) or untreated beds

Design

Preclinical

Authors

TMTakayuki MatsumotoSOShu-ich OdaTKTsuneo Kobayashi

Discussion

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Overview

Rat data implicate NO-ET-1 interplay in flow-mediated mesenteric tone; leaves open translation to human endothelial dysfunction.

Structured PICO

P
Population
Isolated mesenteric arterial beds from 18-week-old male Wistar rats used to evaluate flow-induced endothelium-dependent vasoreactivity.
I
Intervention
Gradually increasing perfusion flow rates (5 to 30 ml/min) to induce shear stress, with or without pharmacological inhibitors (L-NOARG, methylene blue, TEA, indomethacin, BQ-123, BQ-788)
C
Comparator
Low flow rate (5 ml/min) or untreated beds
O
Outcome
Changes in perfusion pressure, ET-1 release, and NO2-/NO3- levelssurrogate

Flow-induced changes in vascular tone in the rat mesenteric arterial bed are subtly regulated by the interaction of endothelium-derived factors including NO, EDHF, and ET-1.

Limitations

  • In vitro animal model, which may not fully replicate in vivo human physiology

Cite This Study

Matsumoto et al. (2004) studied Healthy (animal model). High perfusion flow rate vs. Low perfusion flow rate (5 ml/min) was evaluated on Perfusion pressure and release of endothelium-derived factors (ET-1, NO). High perfusion flow in rat mesenteric arterial beds increased perfusion pressure and stimulated the release of NO and ET-1, demonstrating that vascular tone is regulated by interacting endothelium-derived factors.

synapsesocial.com/papers/6a9b19d0cef11090e02616dahttps://doi.org/10.1540/jsmr.40.1
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Flow-Dependent Regulation of Nitric Oxide Formation in the Isolated Canine Mesenteric Arterial Bed1997 · 1 citations
  2. 2Flow-Dependent Regulation of Nitric Oxide Formation in the IsolatedCanine Mesenteric Arterial Bed.1997 · 5 citations
  3. 3Involvement of endothelium‐derived NO in the basal tone and in the vasodilator responses to muscarinic agonists in the rat isolated mesenteric arterial bed1994 · 4 citations
  4. 4<b>Nitric Oxide In Mesenteric Vascular Reactivity: A Comparison Between Rats With Normotension And Hypertension</b>2002 · 43 citations
  5. 5Flow stimulates endothelial cells to release a nitrovasodilator that is potentiated by reduced thiol1990 · 258 citations