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June 1, 1996Molecular EndocrinologyOpen Access

Identification of a growth hormone-responsive STAT5-binding element in the rat insulin 1 gene.

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Population

Rat insulinoma cell line RIN-5AH and COS7 cells

Design

Preclinical

Key result

Growth hormone stimulated rat insulin 1 promoter activity 2-fold by inducing the binding of STAT5 to a gamma-interferon-activated sequence-like element (Ins-GLE).

Authors

EGElisabeth D. GalsgaardFGFabrice GouilleuxBGBernd Groner

Discussion

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Overview

May link GH to insulin transcription via STAT5; leaves open relevance to human islets or glucose homeostasis.

Structured PICO

P
Population
Rat insulinoma cell line RIN-5AH and COS7 cells
I
Intervention
Growth hormone (GH)
O
Outcome
Insulin mRNA levels and rat insulin 1 promoter activitysurrogate

Main Result

Effect estimate: 2-fold increase

Growth hormone stimulates insulin 1 promoter activity by inducing the binding of the transcription factor STAT5 to a specific element (Ins-GLE) in the rat insulin 1 gene.

Cite This Study

Galsgaard et al. (1996) studied this question. Growth hormone (GH) was evaluated on Rat insulin 1 promoter activity (2-fold increase). Growth hormone stimulated rat insulin 1 promoter activity 2-fold by inducing the binding of STAT5 to a gamma-interferon-activated sequence-like element (Ins-GLE).

synapsesocial.com/papers/6a9ed0ae8f37065ff4e98823https://doi.org/10.1210/mend.10.6.8776725
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