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November 1, 1999Arthritis & Rheumatism

A chimeric antibody with the human ?1 constant region as a putative standard for assays to detect IgG ?2-glycoprotein I-dependent anticardiolipin and anti-?2-glycoprotein I antibodies

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Key result

A chimeric antibody (HCAL) demonstrated reactivity to cardiolipin and beta2-glycoprotein I similar to existing standards across 18 independent laboratories, supporting its use as a standard assay.

Population

Mouse myeloma cell line transfected with a construct containing human gamma1 and kappa constant-region genes…

Comparison

Chimeric antibody HCAL vs Standards for IgG aCL and anti-beta2GPI antibody…

Design

Preclinical

Authors

KIKenji IchikawaATAkito TsutsumiTATatsuya Atsumi

Discussion

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Overview

May support APS assay standardization in research; leaves open clinical adoption without human validation.

Structured PICO

P
Population
Mouse myeloma cell line transfected with a construct containing human gamma1 and kappa constant-region genes and variable-region genes of WBCAL-1 (from an APS-prone mouse)
I
Intervention
Chimeric antibody HCAL
C
Comparator
Standards for IgG aCL and anti-beta2GPI antibody assays from 18 independent laboratories
O
Outcome
Reactivity of HCAL to cardiolipin and beta2-glycoprotein I (beta2GPI)surrogate

The chimeric antibody HCAL demonstrates reactivity similar to human aCL and can serve as a standardized reference for IgG aCL and anti-beta2GPI antibody assays in antiphospholipid syndrome.

Cite This Study

Ichikawa et al. (1999) studied Antiphospholipid syndrome. Chimeric antibody HCAL vs. Standards for IgG aCL and anti-beta2GPI antibody assays was evaluated on Reactivity to cardiolipin and beta2-glycoprotein I. A chimeric antibody (HCAL) demonstrated reactivity to cardiolipin and beta2-glycoprotein I similar to existing standards across 18 independent laboratories, supporting its use as a standard assay.

synapsesocial.com/papers/6a9f9de53b2b802f39268cb4https://doi.org/10.1002/1529-0131(199911)42:11<2461::aid-anr25>3.0.co;2-o
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1β<sub>2</sub>‐Glycoprotein i reactivity of monoclonal anticardiolipin antibodies from patients with the antiphospholipid syndrome1994 · 149 citations
  2. 2Anticardiolipin antibodies recognize beta 2-glycoprotein I structure altered by interacting with an oxygen modified solid phase surface.1994 · 531 citations
  3. 3The fifth domain of <i>beta</i> 2-glycoprotein I contains a phospholipid binding site (Cys281-Cys288) and a region recognized by anticardiolipin antibodies.1994 · 273 citations
  4. 4Molecular definition of human β2-glycoprotein (α2-GPI) by cDNA cloning and inter-species differences of β2-GPI in alternation of anticardiolipin binding1991 · 86 citations
  5. 5Heterogeneity of anticardiolipin antibodies defined by the anticardiolipin cofactor1992 · 327 citations