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April 18, 2017Food ChemistryOpen Access

The feasibility of harmonizing gluten ELISA measurements

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Key result

Harmonizing gluten ELISA assays using a common calibrator reduced between-assay variability, but bias caused by differences in assay selectivity remained unaffected.

Population

Gluten ELISA assays/measurements

Comparison

Harmonization strategies including a common… vs Routine ELISA methods without harmonization

Design

Other

Authors

MRMalgorzata RzychonMBMarcel BrohéeFCFernando Cordeiro

Discussion

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Overview

ELISA variability undermines reliable EU gluten-free enforcement; leaves open need for consensus markers beyond reference materials.

Structured PICO

P
Population
Gluten ELISA assays/measurements
I
Intervention
Harmonization strategies including a common extraction procedure, a common calibrator (pure gluten extract), and an incurred matrix material
C
Comparator
Routine ELISA methods without harmonization
O
Outcome
Comparability of measurements and between-assay variabilitysurrogate

Harmonizing gluten ELISA measurements using reference materials alone is insufficient due to differences in assay selectivity, requiring consensus on robust markers.

Limitations

  • Comparability of measurements is limited by a weak correlation between kit results caused by differences in the selectivity of the methods.
  • Lack of correlation produces bias that cannot be corrected by using reference materials alone.

Cite This Study

Rzychon et al. (2017) studied Gluten measurement. Harmonization of gluten ELISA assays (common extraction procedure, common calibrator) vs. Routine ELISA methods was evaluated on Comparability of measurements and between-assay variability. Harmonizing gluten ELISA assays using a common calibrator reduced between-assay variability, but bias caused by differences in assay selectivity remained unaffected.

synapsesocial.com/papers/6a9fc004857570eec471dcb3https://doi.org/10.1016/j.foodchem.2017.04.092
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