Key result
In vitro calf kidney incubation for ≥24 hours supports bovine enterovirus replication without cell division.
The development of sensitivity to bovine enterovirus infection in calf kidney tissue correlates with the appearance of receptor materials, independent of new cell growth.
Bovine enterovirus receptor emergence in calf kidney occurs without mitosis; leaves open relevance to natural infection or other hosts.
Bovine enterovirus (BE-1) replicates well in primary cell cultures of calf kidney within 24 hr after inoculation. Debris (2.5-20%) prepared from such cell cultures inactivates greater than 90% of the infectivi-ty of a BE-1 inoculum. Freshly excised calf kidney will support the replication of BE-1 within 24 hr only if incubated in vitro for 24 hr or longer prior to virus inoculation. Correlated with the development of sensitivity to virus infection, is the appearance of virus inactivating materials (receptors) in homogenates prepared from the incubated mince. The development of virus sensitivity and receptor material presumably was not due to the growth of new cells, since similar events occurred when cell division was inhibited by the incorporation of colchicine into the maintenance medium.
No takes yet. Share an insight, caveat, or question.
Casto et al. (1969) studied Bovine enterovirus infection (in vitro). In vitro incubation of freshly excised calf kidney vs. Freshly excised calf kidney without prior incubation was evaluated on Replication of BE-1 and appearance of virus inactivating materials (receptors). In vitro incubation of freshly excised calf kidney for ≥24 hours supported bovine enterovirus replication and receptor development, which occurred even when cell division was inhibited.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: