What question did this study set out to answer?

To evaluate the distribution and synthesis of von Willebrand factor across different vascular beds in normal young and adult pigs compared with pigs affected by von Willebrand disease.

January 1, 1987Arteriosclerosis An Official Journal of the American Heart Association Inc

Differential distribution of von Willebrand factor in endothelial cells. Comparison between normal pigs and pigs with von Willebrand disease.

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To evaluate the distribution and synthesis of von Willebrand factor across different vascular beds in normal young and adult pigs compared with pigs affected by von Willebrand disease.
Cultured endothelial cells isolated from the thoracic aorta, inferior vena cava, and pulmonary artery of normal adult pigs, young pigs, and pigs with von Willebrand disease.
Measured von Willebrand factor levels in cell lysates and culture supernatants using ELISA and visualized intracellular presence via immunofluorescence with polyclonal and monoclonal antibodies.
Normal adult pigs exhibited the highest von Willebrand factor concentrations in pulmonary artery endothelial cells, intermediate levels in the inferior vena cava, and nearly undetectable levels in the thoracic aorta.
Normal young pigs exhibited higher von Willebrand factor levels in inferior vena cava endothelial cells than in pulmonary artery cells, demonstrating age-related modulation of factor synthesis.
Endothelial cells from pigs with von Willebrand disease had weakly detectable von Willebrand factor in the pulmonary artery despite plasma levels below 0.01 U/ml, but completely undetectable levels in the thoracic aorta and inferior vena cava.

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Implication

Comparative animal study demonstrates vascular-bed and age-dependent variation of von Willebrand factor in porcine endothelial cells, indicating atherogenesis relates to circulating forms.

Cite This Study

Wu et al. (1987) studied this question.

synapsesocial.com/papers/6aa2a1fca841a277b27f69ed https://doi.org/10.1161/01.atv.7.1.47

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Implication

Comparative animal study demonstrates vascular-bed and age-dependent variation of von Willebrand factor in porcine endothelial cells, indicating atherogenesis relates to circulating forms.

Differential distribution of von Willebrand factor in endothelial cells. Comparison between normal pigs and pigs with von Willebrand disease.

Key Points

Discussion

Implication

Cite This Study

Differential distribution of von Willebrand factor in endothelial cells. Comparison between normal pigs and pigs with von Willebrand disease.

Key Points

Discussion

Implication

Cite This Study

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