Key result
Direct ELISA detects 41 ng/ml of Ren-2 renin in AKR mouse kidneys but not BALB/c controls.
The study demonstrates the presence of both pro- and active forms of Ren-2 renin in the kidney and plasma of two-renin gene mice using a novel ELISA method.
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Remains limited to animal models; leaves open whether Ren-2 ELISA informs human hypertension research.
Ren-1 renin is synthesized in the kidney of every mouse. Ren-2 renin has been observed in the submandibular gland (SMG) of male mice carrying two renin genes. However, it is not known if Ren-2 renin is in the kidney and blood of the two-renin gene mice. In this study, a direct ELISA for Ren-2 renin (SMG renin) was established by a sandwich method. This ELISA could measure the Ren-2 active renin in the range from 1 to 100 ng and distinguish Ren-2 active from not only Ren-1 renin but also Ren-2 prorenin. By a combination of this assay system and conventional methods, the pro-form as well as the active form of Ren-2 renin was found in the kidney and plasma of male AKR mice carrying two-renin genes.
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Suzuki et al. (1990) studied Healthy mice (basic science). Two-renin gene strain (AKR mice) vs. One-renin gene strain (BALB/c mice) was evaluated on Ren-2 renin concentration in kidney extract. Ren-2 active renin and its prorenin were successfully detected in the kidney (41 ng/ml) and plasma (11 ng/ml) of male AKR mice carrying two renin genes, whereas it was undetectable in BALB/c mice.
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