Key result
Trypsin at 2000 mcg/ml maximizes inactive renin activation in human plasma independent of anti-tryptic activity.
Why the study?
The optimum concentration of trypsin for maximum activation of inactive renin in human plasma and its relationship with endogenous anti-tryptic activity were unclear.
Population
Plasma samples from 31 normal subjects
Comparison
Trypsin at 500, 1000, 2000, 3000, and 4000 microgram/ml
Design
Preclinical experimental study
Authors
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Optimizes in vitro renin activation protocols; leaves open clinical translation pending prospective validation.
The optimal concentration of trypsin for activating inactive renin in human plasma in vitro is 2000 microgram/ml, which is independent of endogenous anti-tryptic activity.
Rappelli et al. (1981) studied Healthy (n=31). Trypsin vs. Different concentrations of trypsin was evaluated on Maximum activation of inactive renin. Maximum activation of inactive renin in human plasma is achieved with 2000 microgram/ml of trypsin, independent of endogenous anti-tryptic activity.
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