Investigating the Inhibitory Effects of Polyphenols on Digestive Enzymes under Simulated Intestinal In Vitro Digestion Conditions

Numerous studies have reported inhibitory effects of polyphenols on digestive enzymes, including pancreatic lipase, α-amylase and sucrase-isomaltase, highlighting their potential for the prevention of obesity and diabetes.However, most studies are conducted under simplified buffer conditions using artificial substrates and isolated enzymes, which are specifically suited to these assay systems but inherently limit physiological relevance.Moreover, such approaches typically rely on endpoint quantification and do not capture hydrolysis kinetics, such as digestion rate and extent of substrate conversion.