Key result
Sequential passage of tomato spotted wilt virus generated defective interfering RNAs that retained both genomic termini and contained a single internal deletion of 60% to 80% of the L RNA segment.
Population
Tomato spotted wilt virus (TSWV) isolates (four distinct isolates)
Design
Preclinical
Authors
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Provides structural insights into TSWV DI RNAs; leaves open their role in modulating viral fitness for future research.
TSWV DI L RNAs retain genomic termini and maintain open reading frames, suggesting a unique generation mechanism involving viral polymerase jumping across internal sequences.
Resende et al. (1992) studied Tomato spotted wilt virus. Sequential passage at high multiplicity was evaluated on Characterization of defective interfering RNAs. Sequential passage of tomato spotted wilt virus generated defective interfering RNAs that retained both genomic termini and contained a single internal deletion of 60% to 80% of the L RNA segment.
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