Research Protocol for Evaluation of Vishaghna Effect of Sukshma Ela Beeja Churna (Elettaria Cardamomum Maton) in Doxorubicin Induced Cardiotoxicity in Wistar Albino Rats

The Drug-induced cardiotoxicity has become a major clinical concern, and according to Ayurveda, prolonged use of certain medicinal formulations may result in the accumulation of Gara Visha, contributing to cardiac disorders. According to Ayurveda, prolonged exposure to such toxic substances can disturb normal physiological functions and may contribute to Hridroga (cardiac disorders). Doxorubicin is a widely used anthracycline chemotherapeutic agent known for its dose-dependent cardiotoxic adverse effects, primarily affecting myocardial cells through oxidative stress, inflammation, and apoptosis. Ayurveda describes toxic manifestations under the concept of Gara Visha, and several drugs possessing Vishaghna (anti-toxic) properties are indicated for the management of toxic conditions. Sukshma Ela (Elettaria cardamomum Maton) included under Vishaghna Dravyas, is traditionally described for its Hrudya (cardioprotective), Deepana and Vishaghna properties. The objective of the current research is design to evaluate the Vishaghna and cardioprotective effect of Sukshma Ela Beeja Churna in doxorubicin-induced cardiotoxicity in Wistar albino rats. In this Animals will divide into four groups comprising Normal control, Disease control, Test group I, Test Group II. Assessment will be performed through serum biochemical parameters CPK-MB and LDH, and histopathological examination of cardiac tissue. The study aims to provide experimental validation for the classical claims of Sukshma Ela as a Vishaghna and to establish its potential cardioprotective effect against doxorubicin-induced myocardial toxicity.