Figure 3 from Loss of Predicted Cell Adhesion Molecule MPZL3 Promotes EMT in Ovarian Cancer

MPZL3 knockdown increases EMT gene expression signature which is associated with poor patient outcome. A, Heatmap of EMT signature gene transcripts increased in response to MPZL3 knockdown with two independent shRNAs in OVCAR4 and OVCA433 cells (RNA-seq, z-scores). B, Overall patient survival decreases with high expression of the MPZL3-dependent EMT gene signature (TCGA ovarian serous adenocarcinoma, log-rank Mantel–Cox test). C, Expression of VIM and N-cadherin protein levels are increased following MPZL3 knockdown in OVCAR4 cells. D, Densitometry quantification of VIM and N-cadherin expression from five independent immunoblot experiments [n = 5; one-way ANOVA P = 0.0115 (MPZL3), P = 0.0065 (N-cadherin), P = 0.0025 (VIM); Dunnett multiple comparison test P values shown]. E, VIM expression is negatively correlated to MPZL3 expression in TCGA ovarian serous adenocarcinoma specimens (mRNA z-scores; RNA-seq V2 RSEM). CI, confidence interval.