Figure 4 from Loss of Predicted Cell Adhesion Molecule MPZL3 Promotes EMT in Ovarian Cancer

Loss of MPZL3 reduces homotypic cell adhesion and induces invasion of ovarian cancer cells. A, Homotypic adhesion was assessed by seeding parental OVCAR4 cells in a monolayer (phase-contrast image) and quantifying adherence of OVCAR4 MPZL3 knockdown cells labeled with Far Red fluorescent relative to the adherence of scr control cells (scale bar, 100 μm; n = 3; one-way ANOVA P = 0.0133; Dunnett multiple comparison test P values shown). B, Heterotypic cell adhesion was assessed as in A, except OVCAR4 scr control or MPZL3 knockdown cells labeled with Far Red fluorescent dye were placed on GFP-labeled mesothelial cells (n = 3; one-way ANOVA P = 0.7023; Dunnett multiple comparison test P values shown). C, GFP-labeled mesothelial cells were seeded in a monolayer, and mesothelial clearance by OVCAR4 scr control or MPZL3 knockdown spheroids labeled with Far Red fluorescent dye was quantified after 48 hours (scale bar, 100 μm; n = 3; one-way ANOVA P = 0.0008; Dunnett multiple comparison test P values shown).