Figure 4 from Mesenchymal Stem Cells Expressing CES1 and Soluble TRAIL Activate CPT-11 and Induce Apoptosis in Lung Cancer Brain Metastatic Lesions

Therapeutic effects of treatment genes on lung cancer brain metastasis in vivo. A, Confirming CES1 and FLT-3L expression in Western blot results of H460-Luc cells transfected with LNP-mRNA. B, Bioluminescence imaging was conducted using IVIS Optical Imaging equipment during the experiment. The bioluminescent image of the first mouse in the mock day 7 condition is also presented for the day 7 condition in Fig. 3A, as the images represent the same experimental conditions. C, Bioluminescent signals were quantified using the IVIS imaging system, and results were obtained at 7 and 14 days after injecting H460-Luc cells for each group. D, Mouse body weight changes after injecting H460-Luc cells with transfection were assessed in each group of nude mice. E, Histologic analysis of tumor tissues in the H460-Luc model, stained with hematoxylin and eosin (H&E), Ki67, and cleaved caspase-3. F, A graph illustrating the area of cancer lesions in the mouse brain. All results are expressed as the mean ± SD; N = 5 for all groups. P values of statistical significance are represented as *, P < 0.05; **, P < 0.001; and ***, P < 0.0001.