Figure 7 from Mesenchymal Stem Cells Expressing CES1 and Soluble TRAIL Activate CPT-11 and Induce Apoptosis in Lung Cancer Brain Metastatic Lesions

Histologic and molecular analyses of therapeutic outcomes in the brain metastasis model. A, Histologic examination of brain tumor tissues stained with hematoxylin and eosin (H&E) and Ki67. B, Quantification of tumor lesion area in the brain. C, Representative images of Ki67 immunostaining in brain tumor lesions. D, Quantification of Ki67-positive cells per group. E–H, Immunofluorescence staining for cleaved caspase-3 and CD31 in brain tissues, visualized using the ScanScope AT system (Leica Biosystems). Immunofluorescence staining for cleaved caspase-3 and CD31 in brain tissues, visualized using the ScanScope AT system (Leica Biosystems). E and F, Representative images and quantification of cleaved caspase-3–positive cells. G and H, Representative images and quantification of CD31-positive cells. Data are presented as the mean ± SD; n = 5 per group. P values of statistical significance are represented as *, P < 0.05; **, P < 0.001; and ***, P < 0.0001. DAPI, 4′,6-diamidino-2-phenylindole, dihydrochloride.