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This is a Quantitative Study study.

October 3, 2025Clinical ChemistryOpen Access

B-028 Absorbance vs. Chemiluminescent: A Comparison of Two Different Enzyme-Immunoassay Methods of Digoxin Measurement

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Retrospective analysis compares absorbance and chemiluminescent enzyme-immunoassays for digoxin, suggesting chemiluminescence offers better accuracy.

Chemiluminescence method exhibited lesser bias and imprecision compared to absorbance method.
Both methods showed comparable mean percent recovery, but chemiluminescence had advantages in calibration frequency.
Regression analyses indicated proportional biases for chemiluminescence but constant biases for absorbance.
This validation indicates differences in immunoassay methods can lead to variations in measurement specifications.

S. Islam (2025) studied this question.

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Retrospective analysis compares absorbance and chemiluminescent enzyme-immunoassays for digoxin, suggesting chemiluminescence offers better accuracy.