Figure S1 from Genetic Control of tRNA-Derived Fragments Contributes to Cancer Risk

Figure S1. Determination of the transfection efficiency. A, Relative expression levels of tRF-18-HSQS52D2 overexpression were determined by qRT-PCR method in CRC HCT116 and SW480 cell lines. These cells were transfected with tRF-18-HSQS52D2-mimics, NC-mimics or were left non-transfected (Vehicle). Data are presented as the mean ± SD from three experiments, each with three replicates and all P-values were calculated by a two-sided Student’s t-test. B, Relative expression levels of POU2F1 knockdown were determined using qRT-PCR method in CRC HCT116 and SW480 cell lines. These cells were transfected with siPOU2F1, siControl or were left non-transfected (Vehicle). Data are presented as the mean ± SD from three experiments, each with three replicates and all P-values were calculated by a two-sided Student’s t-test. ns, not significant.