Do antibodies raised against mouse renin reduce blood pressure in anaesthetized rats compared to converting enzyme inhibition?
Antibodies against mouse renin effectively inhibit rat renin and reduce blood pressure in rats to a similar extent as converting enzyme inhibition.
Antibodies raised against mouse submaxillary renin showed affinity for rat renal renin. The affinity was characterized by enzyme kinetics in vitro. The antibodies and the purified Fab fragments showed total inhibition of rat renin, with a Ki of 6.5 +/- 0.7 x 10(-8)mol/l for the Fab fragments. The effect of enzymatic inhibition on blood pressure in anaesthetized rats was compared with converting enzyme inhibition. The antibodies showed a significant effect on blood pressure, with a depressor effect corresponding to that of the converting enzyme inhibitor SQ 20 881. The reduction of mean arterial pressure was from 116 +/- 3 to 108 +/- 3 mmHg for the antibodies and to 107 +/- 4 mmHg for the converting enzyme inhibitor. The pressor response of infused rat renal renin was totally blocked by the antibodies while pressor responses of angiotensin II were unchanged.
Lykkegård et al. (Mon,) studied this question.