What does this research mean for the field?

L-lactate, pyruvate, and certain other organic acids enter sheep cardiac Purkinje cells via facilitated diffusion, causing faster intracellular acidification than predicted by their dissociation constants and lipid solubility. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This research explores how various organic acids affect intracellular pH in sheep cardiac Purkinje strands.

September 1, 1983AJP Cell Physiology

Influence of organic acids on intracellular pH

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Population

Isolated sheep cardiac Purkinje strands

Comparison

Equimolar substitution of 20 mmol/l Cl- by… vs Other organic acids or absence of…

Design

Preclinical

Authors

AHA. de HemptinneVrije Universiteit Brussel RMRoger MarrannesAZ Turnhout BVBert VanheelGhent University Hospital

Discussion

Member takes

Implication

Caution against extrapolating sheep Purkinje cell findings to human therapy; extends evidence for monocarboxylate facilitated diffusion in cardiac tissue.

Key Points

This research explores how various organic acids affect intracellular pH in sheep cardiac Purkinje strands.
Intracellular pH was measured using double-barreled pH-sensitive microelectrodes.
Isolated sheep cardiac Purkinje strands were used for the measurements.
Different organic acids were substituted into the solution to observe their acidification effects.
L-lactate and pyruvate induced faster intracellular acidification than expected from their properties (pK'a, p').
The presence of alpha-cyano-4-hydroxycinnamate significantly reduced acidification rates for certain organic acids.
L-lactate caused faster acidification than D-lactate, indicating the selective transport of L-lactate.

Structured PICO

Population

Isolated sheep cardiac Purkinje strands

Intervention

Equimolar substitution of 20 mmol/l Cl- by organic anions (including L-lactate and pyruvate) at constant extracellular pH 6.8, with or without 4 mmol/l alpha-cyano-4-hydroxycinnamate

Comparator

Other organic acids (e.g., propionate, acetate, D-lactate) or absence of alpha-cyano-4-hydroxycinnamate

Outcome

Rate of induced intracellular acidificationsurrogate

The study provides evidence for facilitated diffusion of L-lactate, pyruvate, and other organic acids in sheep cardiac Purkinje cells.

Cite This Study

Hemptinne et al. (1983) studied this question.

synapsesocial.com/papers/6a1d6d241c2cbcb15c5e49db https://doi.org/10.1152/ajpcell.1983.245.3.c178

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Implication

Caution against extrapolating sheep Purkinje cell findings to human therapy; extends evidence for monocarboxylate facilitated diffusion in cardiac tissue.