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August 24, 2007Ultrasound in Obstetrics and GynecologyOpen Access

Systolic and diastolic ventricular function in the normal and extra‐embryonic venous clipped chicken embryo of stage 24: a pressure–volume loop assessment

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Key result

Venous obstruction by clipping significantly reduced end-systolic elastance (2.91 vs 7.53 mmHg/microL, P<0.005) and increased diastolic stiffness in stage-24 chicken embryos.

Why the study?

Does permanent obstruction of the right lateral vitelline vein alter intrinsic systolic and diastolic ventricular function in stage-24 chicken embryos?

Population

in-ovo stage-24 chicken embryos (n=30)

Comparison

Permanent obstruction of the right lateral… vs Control embryos

Design

Preclinical

Follow-up

2 days

Authors

SVSandra Stekelenburg‐de VosPSPaul SteendijkNUN. T. C. Ursem

Discussion

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Overview

Embryonic venous obstruction impairs ventricular function; leaves open mechanistic role in human congenital heart disease.

Structured PICO

Does permanent obstruction of the right lateral vitelline vein alter intrinsic systolic and diastolic ventricular function in stage-24 chicken embryos?

P
Population
30 in-ovo stage-24 chicken embryos (15 venous clipped, 15 controls) assessed for intrinsic systolic and diastolic ventricular function.
I
Intervention
Permanent obstruction of the right lateral vitelline vein by clipping at 2.5 days of incubation (stage 17)
C
Comparator
Control embryos
O
Outcome
Intrinsic systolic and diastolic ventricular function determined by pressure-volume loop analysissurrogate

Main Result

Absolute Event Rate: 2.91% vs 7.53%

p-value: p=<0.005

Venous obstruction in chicken embryos impairs intrinsic systolic and diastolic ventricular function, which may precede morphological derangements in cardiac development.

Cite This Study

Vos et al. (2007) studied Cardiac development in chicken embryos (n=30). Venous obstruction by clipping the right lateral vitelline vein vs. Control embryos was evaluated on End-systolic elastance (slope of end-systolic pressure-volume relationship) (p=<0.005). Venous obstruction by clipping significantly reduced end-systolic elastance (2.91 vs 7.53 mmHg/microL, P<0.005) and increased diastolic stiffness in stage-24 chicken embryos.

synapsesocial.com/papers/6a20216e6ed993cb49fbed3dhttps://doi.org/10.1002/uog.5137
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Marked Discordance Between Dynamic and Passive Diastolic Pressure-Volume Relations in Idiopathic Hypertrophic Cardiomyopathy1996 · 137 citations
  2. 2The use of left ventricular end-ejection pressure and peak pressure in the estimation of the end-systolic pressure-volume relationship.1984 · 195 citations
  3. 3Transmural distribution of intramyocardial pressure measured by micropipette technique1985 · 100 citations
  4. 4Blood flow and vascular gene expression: fluid shear stress as a modulator of endothelial phenotype1999 · 389 citations
  5. 5Acutely altered hemodynamics following venous obstruction in the early chick embryo2003 · 61 citations