Total ischemia in dog hearts, in vitro. 1. Comparison of high energy phosphate production, utilization, and depletion, and of adenine nucleotide catabolism in total ischemia in vitro vs. severe ischemia in vivo.

Robert B. Jennings; K A Reimer; M L Hill; Steven E. Mayer

doi:10.1161/01.res.49.4.892

October 1, 1981Circulation ResearchOpen Access

Total ischemia in dog hearts, in vitro. 1. Comparison of high energy phosphate production, utilization, and depletion, and of adenine nucleotide catabolism in total ischemia in vitro vs. severe ischemia in vivo.

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Why the study?

Does total ischemia in vitro accurately model severe ischemia in vivo in terms of high energy phosphate metabolism in dog hearts?

Key Points

This study aims to compare the rates of high energy phosphate (HEP) utilization and catabolic products between in vitro total ischemia and in vivo severe ischemia in canine hearts.
Conducted in vitro experiments on dog hearts to analyze high energy phosphate production and utilization during total ischemia.
Assessed the rates of ATP depletion and the accumulation of myocardial lactate during both total and severe ischemia.
Measured changes in adenine nucleotide catabolism and metabolic byproducts over time under different ischemic conditions.
HEP utilization was significantly faster during the first 15 minutes of severe ischemia in vivo compared to total ischemia in vitro.
ATP levels decreased from 5-6 µmol/g to 1.0 µmol/g in 75 minutes of total ischemia in vitro, while similar levels were reached within 30 minutes in vivo.
More than 80% of utilized HEP was derived from anaerobic glycolysis in both ischemia models, indicating comparable metabolic pathways.

Structured PICO

Does total ischemia in vitro accurately model severe ischemia in vivo in terms of high energy phosphate metabolism in dog hearts?

P

Population

Dog hearts

I

Intervention

Total ischemia in vitro

C

Comparator

Severe ischemia in vivo

O

Outcome

Rates of high energy phosphate (HEP) utilization and depletion, and production and distribution of catabolic products of adenine nucleotidessurrogate

Total ischemia in vitro is a valid model for severe ischemia in vivo, demonstrating similar pathways of energy production and nucleotide degradation but with a slower time course.

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Implication

Supports in vitro models for ischemic metabolism pathways in dogs; leaves open translation of ATP kinetics to clinical ischemia.

Abstract

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Cite This Study

Jennings et al. (1981) studied this question.

synapsesocial.com/papers/6a211d664e039c92ec9d0b77 https://doi.org/10.1161/01.res.49.4.892

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Total ischemia in dog hearts, in vitro. 1. Comparison of high energy phosphate production, utilization, and depletion, and of adenine nucleotide catabolism in total ischemia in vitro vs. severe ischemia in vivo.

Key Points

Structured PICO

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Implication

Abstract

Discussion

Cite This Study

Total ischemia in dog hearts, in vitro. 1. Comparison of high energy phosphate production, utilization, and depletion, and of adenine nucleotide catabolism in total ischemia in vitro vs. severe ischemia in vivo.

Key Points

Structured PICO

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Implication

Abstract

Discussion

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