What question did this study set out to answer?

This research aims to determine if ANF and its prohormones are secreted simultaneously in response to atrial distension.

June 1, 1991AJP Heart and Circulatory Physiology

Release of ANF, proANF 1-98, and proANF 31-67 from isolated rat atria by atrial distension

View Full Paper

Ask AI

Bookmark

Why the study?

Does atrial distension increase the secretion of ANF and proANF peptides in isolated rat atria?

Population

Isolated paced left atria from rats (n=15 total; n=9 in distension group, n=6 in control group)

Comparison

Atrial distension vs Maintenance of atrial pressure at approximately…

Design

Preclinical

Follow-up

120 minutes

Authors

JDJohn R. DietzDartmouth College SNS. J. NazianUniversity of South Florida DVDavid L. VeselyArkansas Children's Hospital

Discussion

Member takes

Implication

Supports co-secretion of ANF and proANF with atrial distension in rat atria; extends mechanistic data but leaves clinical translation open.

Key Points

This research aims to determine if ANF and its prohormones are secreted simultaneously in response to atrial distension.
Isolated rat atria were perfused with modified Krebs buffer.
Atrial pressure was increased from 2 mmHg to 8-9 mmHg for 60 minutes in one group (n=9).
Secretion levels of ANF, proANF 31-67, and proANF 1-98 were measured before, during, and after distension.
ANF secretion increased from 100 pg/min to 600-800 pg/min (P<0.001) during distension.
Secretion of proANF 31-67 increased from 100 pg/min to over 200 pg/min (P<0.01).
ProANF 1-98 secretion increased from 1.25 ng/min to 2.5 ng/min (P<0.01) during distension.

Structured PICO

Does atrial distension increase the secretion of ANF and proANF peptides in isolated rat atria?

Population

Isolated paced left atria from rats (n=15 total; n=9 in distension group, n=6 in control group)

Intervention

Atrial distension (increasing atrial pressure from 2 mmHg to 8-9 mmHg for 60 minutes)

Comparator

Maintenance of atrial pressure at approximately 2 mmHg throughout the experimental period

Outcome

Secretion rates of ANF, proANF 1-98, and proANF 31-67surrogate

Atrial distension simultaneously stimulates the secretion of ANF and the NH2-terminus of the ANF prohormone in isolated rat atria.

Cite This Study

Dietz et al. (1991) studied this question.

synapsesocial.com/papers/6a228dfdf56da133636d0663 https://doi.org/10.1152/ajpheart.1991.260.6.h1774

View Full Paper

Ask AI

Bookmark

Implication

Supports co-secretion of ANF and proANF with atrial distension in rat atria; extends mechanistic data but leaves clinical translation open.

Release of ANF, proANF 1-98, and proANF 31-67 from isolated rat atria by atrial distension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Release of ANF, proANF 1-98, and proANF 31-67 from isolated rat atria by atrial distension

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider