What is the clinical evidence from this study?

Study design: Other. Population: Poliovirus infection. Intervention: Poliovirus infection vs. Uninfected HeLa cells. Primary outcome: Structural and functional alterations of the cap-binding protein complex (CBPC).

What question did this study set out to answer?

This research aims to investigate the structural and functional changes in the cap-binding protein complex (CBPC) in HeLa cells infected with poliovirus.

October 1, 1987Journal of Biological ChemistryOpen Access

The cap-binding protein complex in uninfected and poliovirus-infected HeLa cells.

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Key result

Poliovirus infection of HeLa cells caused major structural alterations in the cap-binding protein complex, including loss of the 220-kDa component, stimulating translation of uncapped viral mRNA.

Population

Uninfected and poliovirus-infected HeLa cells

Comparison

Poliovirus infection vs Uninfected HeLa cells

Design

Preclinical

Authors

BBBecky BuckleyTexas Medical Center EEE EhrenfeldUniversity of Utah

Discussion

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Implication

CBPC defect impairs host mRNA translation in poliovirus infection; leaves open specific polypeptide targets for further mechanistic studies.

Key Points

This research aims to investigate the structural and functional changes in the cap-binding protein complex (CBPC) in HeLa cells infected with poliovirus.
Purified CBPC and polypeptide components from uninfected and poliovirus-infected HeLa cells.
Analyzed polypeptide composition and structural alterations in the CBPC.
Conducted in vitro translation assays to assess translation capabilities of capped versus uncapped mRNAs.
CBPC from uninfected cells consists of stable 24- and 220-kDa components; however, the 220-kDa component is absent in infected cells.
Modified CBPC from infected cells shows increased presence of degradation products and a labile association with components.
In vitro translation assays reveal that modified CBPC preferentially stimulates translation of uncapped poliovirus mRNA over capped host mRNAs.

Structured PICO

Population

In vitro study of uninfected and poliovirus-infected HeLa cells.

Exposure

Poliovirus infection

Comparator

Uninfected HeLa cells

Outcome

Structural and functional alterations of the cap-binding protein complex (CBPC)surrogate

Poliovirus infection alters the cap-binding protein complex by degrading its 220-kDa component, shifting translation from capped host mRNAs to uncapped viral mRNAs.

Cite This Study

Buckley et al. (1987) studied Poliovirus infection. Poliovirus infection vs. Uninfected HeLa cells was evaluated on Structural and functional alterations of the cap-binding protein complex (CBPC). Poliovirus infection of HeLa cells caused major structural alterations in the cap-binding protein complex, including loss of the 220-kDa component, stimulating translation of uncapped viral mRNA.

synapsesocial.com/papers/6a657c99a6c99762add5f417 https://doi.org/10.1016/s0021-9258(19)76470-9

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Implication

CBPC defect impairs host mRNA translation in poliovirus infection; leaves open specific polypeptide targets for further mechanistic studies.

The cap-binding protein complex in uninfected and poliovirus-infected HeLa cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The cap-binding protein complex in uninfected and poliovirus-infected HeLa cells.

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider