The 26,000-M(r) cap-binding protein localizes to the postribosomal supernatant and ribosomal salt wash in HeLa cells, but the S-200 fraction protein cannot restore translation in poliovirus-infected extracts.
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Cap-binding protein localization and activity in HeLa extracts provide biochemical clues; leaves open its role in poliovirus translation shutoff.
The 26,000-M(r) cap-binding protein was analyzed by a cross-linking assay in cell fractions from uninfected and poliovirus-infected HeLa cells. Cap-binding protein was found in the postribosomal supernatant (S-200) and in the ribosomal salt wash. The cap-binding protein in the S-200 had a sedimentation coefficient of 5 to 7S and lacked the ability to restore translation in extracts of poliovirus-infected cells.
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Hansen et al. (1982) studied this question.
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