The study identifies 20 and 115 kDa nuclear cap binding proteins in HeLa cells, which may play a role in nuclear events such as mRNA splicing or transport.
Answers are grounded in this paper and cite their sources.
Hypothesis-generating for nuclear mRNA events; leaves open roles in splicing, transport, and viral resistance.
Two polypeptides of apparent molecular mass of 20 and 115 kilodaltons in nuclear fractions from HeLa cells were shown to recognize and be crosslinked to the cap structure of eukaryotic mRNAs in a cap-dependent fashion. Crosslinking of the 20 and 115 kDa polypeptides was sensitive to inhibition by low concentrations of the cap analogue m7GDP and resistant to inhibition by high KCl concentrations. In addition, crosslinking of these polypeptides to the cap structure occurred in nuclear extracts prepared from poliovirus-infected cells, under conditions where cytoplasmic cap binding proteins were incapable of interacting with the mRNA cap structure. The possible function of nuclear cap binding proteins is discussed.
No takes yet. Share an insight, caveat, or question.
Rozen et al. (1987) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: