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July 1, 1996Cardiovascular ResearchOpen Access

A possible mechanism for large stretch-induced increase in Ca2+i in isolated guinea-pig ventricular myocytes

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Key result

Stretch-induced increase in intracellular calcium in guinea-pig ventricular myocytes is modulated by extracellular calcium sources and sensitive to verapamil, but not ryanodine or TTX.

Population

Isolated guinea-pig ventricular myocytes loaded with the fluorescent calcium indicator Indo-1

Comparison

Mechanical stretch using carbon fibres, with and… vs Unstretched state or absence of pharmacological…

Design

Preclinical

Authors

FGFrançois GannierEWE. WhiteDGD. Garnier

Discussion

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Overview

Identifies streptomycin-sensitive calcium entry in stretched myocytes; leaves open relevance to human diastolic dysfunction or arrhythmias.

Structured PICO

P
Population
Isolated guinea-pig ventricular myocytes loaded with the fluorescent calcium indicator Indo-1
I
Intervention
Mechanical stretch using carbon fibres, with and without removal of extracellular Ca2+, verapamil (1 μM), ryanodine (1 μM), or TTX (30 μM), alongside computational modeling (OXSOFT HEART version 4)
C
Comparator
Unstretched state or absence of pharmacological blockers
O
Outcome
Intracellular calcium activity ([Ca2+]i) and sarcomere lengthsurrogate

Stretch-induced increases in resting intracellular calcium in ventricular myocytes are driven by extracellular calcium influx via stretch-activated and calcium-window currents, rather than release from intracellular stores.

Cite This Study

Gannier et al. (1996) studied this question. Stretch vs. Baseline / Unstretched state was evaluated on Stretch-induced increase in internal calcium activity ([Ca2+]i). Stretch-induced increase in intracellular calcium in guinea-pig ventricular myocytes is modulated by extracellular calcium sources and sensitive to verapamil, but not ryanodine or TTX.

synapsesocial.com/papers/6a659ca4fbf3afd72ce90518https://doi.org/10.1016/s0008-6363(95)00153-0
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Stretch‐induced increase of resting intracellular calcium concentration in single guinea‐pig ventricular myocytes1991 · 70 citations
  2. 2Streptomycin and intracellular calcium modulate the response of single guinea‐pig ventricular myocytes to axial stretch2003 · 82 citations
  3. 3Effects of Mechanical Stretch on Membrane Currents of Single Ventricular Myocytes of Guinea-Pig Heart.1992 · 115 citations
  4. 4The effects of increasing cell length on auxotonic contractions; membrane potential and intracellular calcium transients in single guinea‐pig ventricular myocytes1993 · 116 citations
  5. 5Effects of mechanical stretch on the membrane potential of guinea pig ventricular muscles.1988 · 17 citations