Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
November 1, 1991Experimental PhysiologyOpen Access

Stretch‐induced increase of resting intracellular calcium concentration in single guinea‐pig ventricular myocytes

View Full Paper
Ask AI
Bookmark
Share

Population

Single guinea-pig ventricular myocytes (n=8 cells)

Comparison

Stretch by carbon fibres vs Pre-stretch levels (resting cell length)

Design

Preclinical

Authors

JGJ.‐Y. Le GuennecUniversité de ToursEWEd WhiteLawrence Livermore National LaboratoryFGFrançois GannierUniversité de Tours

Discussion

Loading...

Member takes

Implication

Stretch elevates myocyte [Ca2+]i reversibly; hypothesis-generating for length-dependent contractility, translation to human hearts untested.

Key Points

  • To determine whether mechanical stretch alters resting intracellular calcium concentration in isolated cardiac ventricular myocytes.
  • Single guinea-pig ventricular myocytes were loaded with the fluorescent calcium indicator Indo-1 AM.
  • Cells were stretched using carbon fibers, producing a 2% to 18% increase in sarcomere length and increased resting tension.
  • Resting intracellular calcium concentration increased upon stretch in 7 out of 8 tested cells.
  • The magnitude of the increase in intracellular calcium correlated with the extent of stretch and reversed to baseline when cells returned to resting length.

Structured PICO

P
Population
Single guinea-pig ventricular myocytes (n=8 cells)
I
Intervention
Stretch by carbon fibres (sarcomere lengths increased by 2-18%)
C
Comparator
Pre-stretch levels (resting cell length)
O
Outcome
Resting intracellular calcium concentration ([Ca2+]i)surrogate

Stretching single ventricular myocytes increases resting intracellular calcium concentration, suggesting a mechanism by which changes in resting muscle length can modify the contractile state of cardiac muscle.

Cite This Study

Guennec et al. (1991) studied this question.

synapsesocial.com/papers/6a8430efc967ec513cbd8e04https://doi.org/10.1113/expphysiol.1991.sp003560
View Full Paper
Ask AI
Bookmark
Share

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1A possible mechanism for large stretch-induced increase in [Ca2+]i in isolated guinea-pig ventricular myocytes1996 · 69 citations
  2. 2The effects of increasing cell length on auxotonic contractions; membrane potential and intracellular calcium transients in single guinea‐pig ventricular myocytes1993 · 116 citations
  3. 3Effects of Mechanical Stretch on Membrane Currents of Single Ventricular Myocytes of Guinea-Pig Heart.1992 · 116 citations
  4. 4The effects of mechanical loading and changes of length on single guinea‐pig ventricular myocytes.1995 · 107 citations
  5. 5Streptomycin and intracellular calcium modulate the response of single guinea‐pig ventricular myocytes to axial stretch2003 · 84 citations