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January 1, 1993Experimental PhysiologyOpen Access

The effects of increasing cell length on auxotonic contractions; membrane potential and intracellular calcium transients in single guinea‐pig ventricular myocytes

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Key result

Stretching single guinea-pig ventricular myocytes by approximately 3% significantly decreased action potential duration (P<0.01) and intracellular calcium transient duration (P<0.025).

Population

Single guinea-pig ventricular myocytes

Comparison

Stretch vs Baseline (before stretch)

Design

Preclinical

Authors

EWEdward WhiteJGJ.‐Y. Le GuennecJNJ. M. Nigretto

Discussion

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Overview

New single-myocyte setup enables simultaneous length, auxotonic, and Ca²⁺ recordings; leaves open translation to intact-heart mechanics.

Structured PICO

P
Population
Single guinea-pig ventricular myocytes studied in an experimental set-up to measure auxotonic contractions, membrane potential, and intracellular calcium transients.
I
Intervention
Stretch (increasing sarcomere length by approximately 3%)
C
Comparator
Baseline (before stretch)
O
Outcome
Auxotonic contractions, membrane potential, and intracellular calcium transientssurrogate

Main Result

p-value: p=<0.01

In single guinea-pig ventricular myocytes, stretching decreases action potential and calcium transient duration without affecting their amplitudes, suggesting time courses are more dependent on tension than length.

Cite This Study

White et al. (1993) studied Guinea-pig ventricular myocytes. Stretch (increasing cell length) vs. Baseline/Unstretched was evaluated on Action potential duration and intracellular calcium transient duration (p=<0.01). Stretching single guinea-pig ventricular myocytes by approximately 3% significantly decreased action potential duration (P<0.01) and intracellular calcium transient duration (P<0.025).

synapsesocial.com/papers/6a659ca4fbf3afd72ce90519https://doi.org/10.1113/expphysiol.1993.sp003671
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1The effects of mechanical loading and changes of length on single guinea‐pig ventricular myocytes.1995 · 107 citations
  2. 2Stretch‐induced increase of resting intracellular calcium concentration in single guinea‐pig ventricular myocytes1991 · 70 citations
  3. 3Effects of Mechanical Stretch on Membrane Currents of Single Ventricular Myocytes of Guinea-Pig Heart.1992 · 115 citations
  4. 4Streptomycin and intracellular calcium modulate the response of single guinea‐pig ventricular myocytes to axial stretch2003 · 82 citations
  5. 5Changes in [Ca2+]i, [Na+]i and Ca2+ current in isolated rat ventricular myocytes following an increase in cell length.1996 · 99 citations