Key result
Hypertensive patients with HFpEF had significantly higher PBMC expression of miR-208b compared to controls (28.8 vs 7.5, P<0.001), which correlated positively with maximal oxygen uptake.
Why the study?
Do microRNA expression levels in peripheral blood mononuclear cells differ in hypertensive patients with HFpEF compared to those without, and do they correlate with exercise capacity?
Observational (n=98)
Do microRNA expression levels in peripheral blood mononuclear cells differ in hypertensive patients with HFpEF compared to those without, and do they correlate with exercise capacity?
Absolute Event Rate: 28.8% vs 7.5%
p-value: p=<0.001
Specific microRNAs (miR-26b, miR-208b, and miR-499) are upregulated in PBMCs of hypertensive patients with HFpEF and correlate with functional capacity, suggesting potential as biomarkers.
miR expression in PBMCs was associated with exercise capacity in hypertensive HFpEF; leaves open their biomarker potential pending prospective validation.
BACKGROUND: MicroRNAs (miRs) regulate gene expression and play an important role in ventricular and vascular remodeling. However, there are limited data regarding their role in heart failure with preserved ejection fraction (HFpEF). The aim of this study was to assess gene expression of miR-1, miR-133a, miR-21, miR-208b, miR-499, and miR-26b in peripheral blood mononuclear cells (PBMCs) in hypertensive patients with HFpEF and to evaluate their association with their exercise capacity. METHODS: We included 56 hypertensive patients with HFpEF (age 67.29 ± 7.75 years). Forty-two hypertensive patients without HFpEF (age 66.83 ± 7.17 years) served as controls. All subjects underwent a cardiopulmonary exercise test (CPXT). PBMCs were isolated and levels of miRs were determined by quantitative real-time reverse transcription polymerase chain reaction. RESULTS: For hypertensive patients with HFpEF, higher expression levels in PBMCs were found only for miR-26b (7.6 ± 7.3 vs. 4.0 ± 3.6, P = 0.002), miR-208b (28.8 ± 35.3 vs. 7.5 ± 13.3, P < 0.001), and miR-499 (14.2 ± 22.4 versus 3.5 ± 2.9, P = 0.001). The strongest correlations with CPXT parameters were found for miR-208b levels, which had a positive correlation with maximal oxygen uptake (peakVO2) (r = 0.671, P < 0.001), exercise duration (r = 0.445, P = 0.001), and minute ventilation-carbon dioxide production relationship (VE/VCO2) (r = 0.437, P = 0.001) in the HFpEF group. CONCLUSIONS: miR-26b, miR-208b, and miR-499 show a distinct in profile in hypertensive patients with HFpEF that is related with functional capacity. Further studies are needed to assess the role of miRs as prognostic tools or as therapeutic targets in those patients.
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Marketou et al. (2018) conducted an observational in Heart failure with preserved ejection fraction in hypertensive patients (n=98). Heart failure with preserved ejection fraction (HFpEF) vs. Hypertensive patients without HFpEF was evaluated on Expression level of miR-208b in peripheral blood mononuclear cells (p=<0.001). Hypertensive patients with HFpEF had significantly higher PBMC expression of miR-208b compared to controls (28.8 vs 7.5, P<0.001), which correlated positively with maximal oxygen uptake.
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