What is the clinical evidence from this study?

Study design: Other. Population: Human rhinovirus 14 (Common cold). Intervention: Nucleotide sequencing. Primary outcome: Complete nucleotide sequence determination.

January 1, 1984Nucleic Acids ResearchOpen Access

The complete nucleotide sequence of a common cold virus: human rhinovlrus 14

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Key result

The complete nucleotide sequence of human rhinovirus 14 was determined, revealing a 7208-nucleotide genome with significant homology to polioviruses.

Population

Human rhinovirus 14 (HRV-14) genome (cDNA cloned in E. coli)

Comparison

Complete nucleotide sequencing vs Polioviruses (for homology comparison)

Design

Preclinical

Authors

GSGlyn StanwayUniversity of Essex PHP. J. HughesUniversity of Essex RMRoger MountfordUniversity of Liverpool

Discussion

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Implication

HRV14 genome sequenced, revealing picornavirus features; extends family genomics but leaves open therapeutic applications.

Key Points

This research aims to determine the complete nucleotide sequence of human rhinovirus 14 and compare it to other viruses.
Determined the complete nucleotide sequence of human rhinovirus 14 from cDNA cloned in E. coli.
Analyzed the 5' non-coding region, open reading frame, and 3' non-coding region of the genome.
Compared nucleotide and predicted amino acid sequences with those of polioviruses.
The genome has a 5' non-coding region of 624 nucleotides, a long open reading frame of 6537 nucleotides, and a 3' non-coding region of 47 nucleotides.
A significant homology was found between human rhinovirus 14 and polioviruses, indicating a genetic relationship.
The findings may help identify regions crucial for virus recognition and predict protein cleavage sites.

Structured PICO

Population

Human rhinovirus 14 (HRV-14) genome (cDNA cloned in E. coli)

Intervention

Complete nucleotide sequencing

Comparator

Polioviruses (for homology comparison)

Outcome

Complete nucleotide sequence and predicted amino acid sequence

The complete nucleotide sequence of human rhinovirus 14 reveals a closer genetic relationship between the rhinovirus and enterovirus genera than previously suspected.

Cite This Study

Stanway et al. (1984) studied Human rhinovirus 14 (Common cold). Nucleotide sequencing was evaluated on Complete nucleotide sequence determination. The complete nucleotide sequence of human rhinovirus 14 was determined, revealing a 7208-nucleotide genome with significant homology to polioviruses.

synapsesocial.com/papers/6a6e82abe5469ee92be0040d https://doi.org/10.1093/nar/12.20.7859

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The complete nucleotide sequence of a common cold virus: human rhinovlrus 14

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

The complete nucleotide sequence of a common cold virus: human rhinovlrus 14

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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