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May 1, 1992Journal of VirologyOpen Access

Structural and functional characterization of the poliovirus replication complex

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Population

poliovirus-infected HEp-2 cells

Design

Preclinical

Authors

KBKurt BienzUniversity of BaselDEDenise EggerUniversity of BernTPThomas D. PfisterRoche (Switzerland)

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Implication

Clarifies poliovirus replication architecture in membranes; leaves open whether these complexes are viable antiviral targets.

Structured PICO

P
Population
poliovirus-infected HEp-2 cells
I
Intervention
Isolation of membrane-bound replication complexes by sucrose gradient centrifugation, treatment with sodium deoxycholate, and in situ hybridization
O
Outcome
Structural and functional characteristics of membrane-bound replication complexes

The study elucidates the architecture of the poliovirus replication complex, suggesting that nascent plus strands are enclosed in compact membranes and replication forks are wrapped in protein.

Cite This Study

Bienz et al. (1992) studied this question.

synapsesocial.com/papers/6a6f55d6a528af2d65c2f068https://doi.org/10.1128/jvi.66.5.2740-2747.1992
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Poliovirus RNA-dependent RNA polymerase and host cell protein synthesize product RNA twice the size of poliovirion RNA in vitro1985 · 68 citations
  2. 2Structural organization of poliovirus RNA replication is mediated by viral proteins of the P2 genomic region1990 · 243 citations
  3. 3Three poliovirus 2B mutants exhibit noncomplementable defects in viral RNA amplification and display dosage-dependent dominance over wild-type poliovirus1991 · 89 citations
  4. 4Isolation of poliovirus 2C mutants defective in viral RNA synthesis1988 · 125 citations
  5. 5An intragenic revertant of a poliovirus 2C mutant has an uncoating defect1990 · 95 citations