Role of tumor necrosis factor-α in myocardial dysfunction and apoptosis during hindlimb ischemia and reperfusion

Xiangru Lu; Joel A. Hamilton; Ji Shen; Theresa Pang; Douglas L. Jones; Richard F. Potter; J. Malcolm O. Arnold; Qingping Feng

doi:10.1097/01.ccm.0000199079.64231.c1

January 17, 2006Critical Care Medicine

Role of tumor necrosis factor-α in myocardial dysfunction and apoptosis during hindlimb ischemia and reperfusion

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Key Points

To determine the role of tumor necrosis factor-alpha in myocardial apoptosis and cardiac dysfunction following hindlimb ischemia and reperfusion.
Subjected mice to 2 hours of bilateral hindlimb ischemia via tourniquet occlusion, followed by reperfusion intervals ranging from 0.5 to 24 hours.
Assessed cardiac dysfunction, myocardial apoptosis via ELISA, TUNEL staining, and caspase-3 activity, and evaluated the therapeutic effect of etanercept in wild-type mice.
Hindlimb ischemia and reperfusion in wild-type mice caused significant early myocardial depression (p < .05) that temporally correlated with elevated myocardial and plasma TNF-α levels, returning to baseline by 24 hours.
Myocardial apoptosis was significantly elevated at 6 hours of reperfusion in wild-type mice (p < .05) before resolving at 24 hours, whereas apoptosis and cardiac dysfunction were completely abolished in TNF-α-/- mice.
Administration of the TNF inhibitor etanercept fully restored cardiac function in wild-type mice subjected to hindlimb ischemia and reperfusion.

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Implication

Randomized animal study demonstrates myocardial dysfunction in mice following hindlimb ischemia reperfusion, highlighting tumor necrosis factor-alpha as a therapeutic target.

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Lu et al. (2006) studied this question.

synapsesocial.com/papers/6a705a5c26a7f98052dcdb52 https://doi.org/10.1097/01.ccm.0000199079.64231.c1

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Role of tumor necrosis factor-α in myocardial dysfunction and apoptosis during hindlimb ischemia and reperfusion

Key Points

No abstract yet

Implication

Discussion

Cite This Study

Role of tumor necrosis factor-α in myocardial dysfunction and apoptosis during hindlimb ischemia and reperfusion

Key Points

No abstract yet

Implication

Discussion

Cite This Study

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