What question did this study set out to answer?

To evaluate the direct actions of atrial natriuretic peptide on arcuate arteries and afferent and efferent arterioles in normal kidney microvasculature.

March 1, 1988AJP Renal Physiology

Direct evaluation of the microvascular actions of ANP in juxtamedullary nephrons

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Population

In vitro blood-perfused juxtamedullary nephrons from normal donor rats pretreated with captopril

Comparison

Topical administration of atriopeptin III at… vs Control conditions and after removal of AP III…

Design

Preclinical

Authors

PVPeter VeldkampUniversity of Pittsburgh PCPamela K. CarminesUniversity of Nebraska Medical Center EIEdward W. InschoUniversity of Alabama at Birmingham

Discussion

Member takes

Implication

ANP selectively dilates preglomerular vessels in normal rat kidney; leaves open translation to human renal hemodynamics or disease.

Key Points

To evaluate the direct actions of atrial natriuretic peptide on arcuate arteries and afferent and efferent arterioles in normal kidney microvasculature.
Utilized the in vitro blood-perfused juxtamedullary nephron technique in captopril-pretreated donor rats to eliminate interactions with angiotensin II.
Conducted videometric analysis of vessel inside diameters during topical superfusion with atriopeptin III (AP III) at concentrations ranging from 0.03 nM to 3 nM.
Superfusion with 3 nM AP III dilated arcuate arteries by 73 ± 9% (baseline: 83 ± 14 µm, n=7) and afferent arterioles by 23 ± 5% (baseline: 20 ± 4 µm, n=7), both returning to baseline after washout.
Arcuate arteries demonstrated concentration-dependent vasodilation, increasing in diameter by 26 ± 9% at 0.3 nM AP III (n=5) with no significant effect at 0.03 nM.
Efferent arteriolar diameter (baseline: 16 ± 2 µm, n=7) was not significantly altered by AP III superfusion.

Structured PICO

Population

In vitro blood-perfused juxtamedullary nephrons from normal donor rats pretreated with captopril

Intervention

Topical administration of atriopeptin III (AP III) at concentrations of 3 nM, 0.3 nM, and 0.03 nM

Comparator

Control conditions (baseline prior to administration) and after removal of AP III from the superfusate

Outcome

Vessel inside diameters of arcuate arteries, afferent arterioles, and efferent arterioles measured by videometric analysissurrogate

Atriopeptin III induces selective preglomerular vasodilation involving arcuate arteries and afferent arterioles without affecting efferent arterioles in the normal rat kidney.

Cite This Study

Veldkamp et al. (1988) studied this question.

synapsesocial.com/papers/6a72a8832163a0a01bc63a77 https://doi.org/10.1152/ajprenal.1988.254.3.f440

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Direct evaluation of the microvascular actions of ANP in juxtamedullary nephrons

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Direct evaluation of the microvascular actions of ANP in juxtamedullary nephrons

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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