What question did this study set out to answer?

To elucidate the mechanism of the adenosine diphosphate (ADP) platelet reaction by examining the effects of specific chemical inhibitors in vitro.

January 1, 1964Thrombosis and Haemostasis

Investigations on Adenosine Diphosphate (ADP) Induced Platelet Adhesiveness in Vitro* Part II. Studies on the Mechanism

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Population

in vitro platelet reaction model

Design

Preclinical

Authors

BSB. A. SkålheggOslo University Hospital AHA. J. HellemOslo University Hospital AÖA. E ÖdegaardOslo University Hospital

Discussion

Member takes

Implication

In vitro platelet inhibition data should not change practice; leaves open translation to clinical antithrombotic strategies.

Key Points

To elucidate the mechanism of the adenosine diphosphate (ADP) platelet reaction by examining the effects of specific chemical inhibitors in vitro.
Assayed ADP-induced platelet adhesiveness in vitro in the presence of chemical inhibitors, including monoiodoacetic acid, molecular iodine, para-chloromercuribenzoic acid, potassium ferricyanide, free -SH compounds, and adenine conjugates.
Assessed in vivo primary bleeding time by flushing open wounds with inhibitor solutions, and evaluated the platelet-aggregating capacity of adenosine tetraphosphate relative to ADP.
Monoiodoacetic acid, molecular iodine, para-chloromercuribenzoic acid, and potassium ferricyanide produced noncompetitive inhibition of the ADP-platelet reaction.
Compounds containing free -SH groups and adenine conjugates produced competitive inhibition and prolonged primary bleeding time when flushed over wounds.
Adenosine tetraphosphate aggregated platelets in an identical manner to ADP, providing insight into the sequential biochemical steps of platelet activation.

Structured PICO

Population

in vitro platelet reaction model

Intervention

Substances which inhibit the ADP platelet reaction (Monoiodo acetic acid, molecular iodine, para-chloro mercuribenzoic acid, potassium ferricyanid, substances containing free -SH groups, and adenine conjugates)

Outcome

Inhibition of ADP-induced platelet adhesiveness and primary bleeding timesurrogate

In vitro studies identify competitive and noncompetitive inhibitors of ADP-induced platelet aggregation, providing mechanistic insights into platelet adhesiveness.

Cite This Study

Skålhegg et al. (1964) studied this question.

synapsesocial.com/papers/6a75c1ed3dd558469eb93453 https://doi.org/10.1055/s-0038-1654831

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Investigations on Adenosine Diphosphate (ADP) Induced Platelet Adhesiveness in Vitro* Part II. Studies on the Mechanism

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Investigations on Adenosine Diphosphate (ADP) Induced Platelet Adhesiveness in Vitro* Part II. Studies on the Mechanism

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider