August 1, 1993Biochemistry

Interaction of polypyrimidine tract binding protein with the encephalomyocarditis virus mRNA internal ribosomal entry site

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Population

Purified polypyrimidine tract binding protein, HeLa cell extracts, and 16 RNA variants derived from the…

Design

Preclinical

Authors

GWGary W. WitherellState University of New York AGAnna GilUniversity of Massachusetts Chan Medical School EWEckard WimmerCalifornia Institute of Technology

Discussion

Member takes

Implication

Offers no clinical application yet; leaves open pPTB targeting in EMCV myocarditis models.

Structured PICO

Population

Purified polypyrimidine tract binding protein (pPTB), HeLa cell extracts, and 16 RNA variants derived from the encephalomyocarditis virus (EMCV) mRNA internal ribosomal entry site (IRES)

Intervention

Interaction characterization using nitrocellulose filter binding and UV cross-linking assays

Outcome

Binding affinity and specificity of pPTB to EMCV IRESsurrogate

The study characterizes the high-affinity, entropy-driven binding of pPTB to the EMCV IRES, which is modulated by other cellular factors for specificity.

Cite This Study

Witherell et al. (1993) studied this question.

synapsesocial.com/papers/6a7a23fefb5b6b43000debcb https://doi.org/10.1021/bi00083a030

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Interaction of polypyrimidine tract binding protein with the encephalomyocarditis virus mRNA internal ribosomal entry site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Interaction of polypyrimidine tract binding protein with the encephalomyocarditis virus mRNA internal ribosomal entry site

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider