March 1, 2000Molecular and Cellular BiologyOpen Access

Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo

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Population

BS-C-1, HeLa, and Huh-7 cell lines transfected with plasmids expressing dicistronic transcripts

Comparison

Expression of wild-type Polypyrimidine-tract… vs Expression of PTB deletion mutants

Design

Preclinical

Authors

RGRainer GosertUniversity Hospital of Basel KCKi Ha ChangUniversity of North Carolina at Chapel Hill RRRené RijnbrandDicerna Pharmaceuticals (United States)

Discussion

Member takes

Implication

Cell culture data should not guide antiviral strategies; leaves open PTB's role in viral IRES translation in animal models.

Structured PICO

Population

BS-C-1, HeLa, and Huh-7 cell lines transfected with plasmids expressing dicistronic transcripts

Intervention

Expression of wild-type Polypyrimidine-tract binding protein (PTB)

Comparator

Expression of PTB deletion mutants (including a null mutant lacking amino acid residues 87 to 531)

Outcome

Reporter protein (chloramphenicol acetyltransferase [CAT]) productionsurrogate

PTB stimulates translation of representative picornaviral and flaviviral RNAs in vivo, likely by stabilizing higher ordered RNA structures within the IRES.

Cite This Study

Gosert et al. (2000) studied this question.

synapsesocial.com/papers/6a7cfe0a3d640844b95e05b2 https://doi.org/10.1128/mcb.20.5.1583-1595.2000

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Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Transient Expression of Cellular Polypyrimidine-Tract Binding Protein Stimulates Cap-Independent Translation Directed by Both Picornaviral and Flaviviral Internal Ribosome Entry Sites In Vivo

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider