Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus
Population
Foot-and-mouth disease virus (FMDV) IRES RNA and rabbit reticulocyte lysate
Comparison
Polypyrimidine tract-binding protein binding and… vs Lysate depleted of internal PTB without addition…
Design
Preclinical
Authors
Discussion
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Member takes
Implication
PTB supports FMDV IRES translation in vitro; leaves open whether PTB targeting alters replication or offers antiviral strategies in vivo.
Structured PICO
P
Population
Foot-and-mouth disease virus (FMDV) IRES RNA and rabbit reticulocyte lysate
I
Intervention
Polypyrimidine tract-binding protein (PTB) binding and addition to depleted lysate
C
Comparator
Lysate depleted of internal PTB without addition of PTB; wild-type PTB-binding site
O
Outcome
Formation of 48S and 80S ribosomal initiation complexes and translation efficiency
PTB plays an important role in the formation of initiation complexes with FMDV IRES RNA and stimulates internal translation initiation.
Cite This Study
Niepmann et al. (1997) studied this question.