September 12, 1994FEBS LettersOpen Access

Pyrimidine tract binding protein strongly stimulates in vitro encephalomyocarditis virus RNA translation at the level of preinitiation complex formation

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Population

Cytoplasmic extracts of Krebs-2 ascites carcinoma cells partially depleted of endogenous PTB

Comparison

Addition of cloned purified PTB vs Extracts without added PTB and translation of…

Design

Preclinical

Authors

Discussion

Preliminary animal data are hypothesis-generating; leaves open PTB relevance to human cardiac IRES-mediated translation.

Population

Cytoplasmic extracts of Krebs-2 ascites carcinoma cells partially depleted of endogenous PTB

Intervention

Addition of cloned purified PTB

Comparator

Extracts without added PTB and translation of capped mRNAs (beta-globin, TMV RNAs)

Outcome

Translation of a beta-glucuronidase (GUS) reporter cistron fused to the EMCV IRES and 48S pre-initiation complex formationsurrogate

PTB strongly stimulates EMCV IRES-directed translation at the level of 48S pre-initiation complex formation.

Borovjagin et al. (1994) studied this question.

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